12th International Meeting on Yeast Apoptosis

Bari, Italy • 14-18 May 2017

 

Programme

  • Sunday, 14 May

    17:00

    Registration opening

    17:30-18:00

    Opening and Welcome

    Chairs: Sergio Giannattasio and Cristina Mazzoni

    Alessandro Ambrosi (President of Bari Chamber of Commerce)

    Giovanni Gorgoni (Special Commissioner of the Regional Health Agency of Apulia - AReS)

    Antonio Felice Uricchio (Rector of the University "Aldo Moro", Bari)

    Graziano Pesole (Director of the Istituto di Biomembrane, Bioenergetica e Biotecnologie Molecolari, IBIOM, Bari)

    18:00-19:00

    Opening Lecture

    Chair: Joris Winderickx

    Frank Madeo (Graz, Austria)
    From dying yeasts to longevity drugs

    19:00-20:00

    Get together party – mingling

  • Monday, 15 May

    8:00-12:00

    Late Registration

    8:30-11:00

    Session 1: Fungal cell death pathways I

    Chairs: Ted Powers and Campbell Gourlay

    8:30-9:00

    Marie Hardwick (Baltimore, MD, USA)
    Yeast cell death and nutrient-sensing pathways merge

    9:00-9:30

    Campbell Gourlay (Canterbury, UK)
    Actin and mitochondria co-ordinate MAPK signalling to control cell health

    9:30-9:48

    Thomas Nystrom (Gothenburg, Sweden)
    Calmodulin regulates protease versus co-chaperone activity of metacaspase - turning an executioner into a protector

    9:48-10:06

    Julianne Grose (Provo, UT, USA)
    PAS kinase: the connection between PASsing glucose and cell death

    10:06-10:24

    Kyle Cunningham (Baltimore, MD, USA)
    Regulation of vacuolar membrane permeabilization in response to membrane stresses

    10:24-10:42

    Elliot Piper-Brown (Canterbury, UK)
    Phosphoregulation of the small GTPase Ras controls cell fate in yeast

    10:42-11:00

    Sreedivya Saladi (Kaiserslautern, Germany)
    Unraveling the role of p1, a yeast AMID homolog, as a key regulator of apoptosis in Saccharomyces cerevisiae

    11:00-11:30

    Coffee break   Sunrise

    11:30-13:00

    Session 2: Fungal cell death pathways II

    Chairs: Manuela Côrte-Real and Katrina Cooper

    11:30-12:00

    Katrina Cooper (Stratford, NJ, USA)
    MAPK and SCF choreograph the 2-step dance to cyclin C mediated cell death

    12:00-12:30

    Manuela Côrte-Real (Braga, Portugal)
    Mechanistic insights on the selective anticancer activity of lactoferrin unveiled by yeast

    12:30-12:48

    Nicanor Austriaco (Providence, RI, USA)
    Yeast Bax inhibitor (Bxi1p/Ybh3p) is a pH-sensitive calcium leak that regulates the microenvironment of the ER in Saccharomyces cerevisiae

    12:48-13:06

    Stefania Francesconi (Paris, France)
    Lack of Mre11 induces apoptosis-like cell death in fission yeast quiescent cells

    13:10-14:00

    Lunch

    14:00-15:00

    Poster session I

    15:00-17:30

    Session 3: Mitochondrial function and pathophysiology

    Chairs: Benedikt Westermann and Fedor Severin

    15:00-15:30

    Benedikt Westermann (Bayreuth, Germany)
    Asymmetric inheritance of mitochondria in yeast

    15:30-16:00

    Michal Eisenberg-Bord (Rehovot, Israel)
    Molecular determinants of a lipid droplet subpopulation at the contact site between the nucleus and vacuole

    16:00-16:18

    Sonia Colombo (Milan, Italy)
    Activated Ras in mitochondria and apoptosis in budding yeast

    16:18-16:36

    Stefan Wölfl (Heidelberg, Germany)
    The many faces of the gluconeogenesis key enzyme FBP1 - Mutations of fructose 1,6 bis-phosphatase changing enzymatic activity, sensitivity to MMS, and nuclear sublocalization

    16:36-16:54

    Fedor Severin (Moscow, Russia)
    Triosephosphates as intermediates of Crabtree effect

    16:54-17:12

    Arianna Montanari (Rome, Italy)
    Mitochondrial diseases: yeast as a model for the study of suppressors

    17:12-17:30

    Cátia Pereira (Braga, Portugal)
    Understanding the interplay between the pro-apoptotic protein lactoferrin and biomembranes: from yeast to human cells

    17:30-18:00

    Coffee break

    18:00-19:40

    Session 4: Metabolic regulation of cell stress response

    Chairs: Dina Petranovic and Sergio Giannattasio

    18:00-18:30

    Jens Nielsen (Gothenburg, Sweden)
    Systems biology of yeast metabolism

    18:30-19:00

    Hiroshi Takagi (Nara, Japan)
    Nitric oxide in yeast: a double-edged sword "cell protection vs. cell death"

    19:00-19:18

    Rita Cha (Bangor, UK)
    Essential DNA-damage-response kinase Mec1ATM/ATR couples metabolic regulation to mitochondrial genome maintenance

    19:18-19:36

    Valentina Cappelletti (Zurich, Switzerland)
    Nutrient-sensing, metabolism and epigenetics: what can we learn from yeast?

    20:30

    Bari old town tour and local products tasting*

    *Separate (free) registration required. Departure strictly at 20:30.

    The event is offered by PugliaPromozione (Programma operativo Regionale FESR-FSE 2014 – 2020 “Attrattori culturali, naturali e turismo” Asse VI – Tutela dell’ambiente e promozione delle risorse naturali e culturali – Azione 6.8.)

  • Tuesday, 16 May

    8:30-10:30

    Session 5: Autophagy/Mitophagy

    Chairs: Francesco Cecconi and Paula Ludovico

    8:30-9:00

    Francesco Cecconi (Copenhagen, Denmark)
    AMBRA1 through evolution at the crossroad among autophagy, cell proliferation and cell death

    9:00-9:30

    Ted Powers (Davis, CA, USA)
    The role of TORC2 signaling and mitochondria in autophagy

    9:30-9:48

    Didac Carmona-Gutierrez (Graz, Austria)
    Identification of a novel autophagy inductor and anti-aging agent

    9:48-10:06

    Paola Coccetti (Milan, Italy)
    Snf1/AMPK regulates metabolism and autophagy in a methionine-dependent manner

    10:06-10:24

    Andreas Aufschnaiter (Graz, Austria)
    Traffic jam: impairment of autophagy and the multivesicular body pathway by the Parkinson’s disease associated proteins α-synuclein and LRRK2

    10:30-11:00

    Coffee break

    11:00-13:00

    Session 6: Cellular ageing

    Chairs: Cristina Mazzoni and Frank Madeo

    11:00-11:30

    Zdena Palková (Prague, Czech Republic)
    Mitochondria, important players in ageing and metabolic differentiation of yeast colonies

    11:30-12:00

    Lynn Megeney (Ottawa, ON, Canada)
    Caspase and metacaspase proteases require distinct regulatory mechanisms to manage non-apoptotic cell function

    12:00-12:18

    David Goldfarb (Rochester, NY, USA)
    Topoisomerase 2 and the DNA damage theory of aging

    12:18-12:36

    Angelina Gross (Graz, Austria)
    Lipid metabolism in the regulation of autophagy and ageing in yeast

    12:36-12:54

    Marija Cvijovic (Gothenburg, Sweden)
    Beyond asymmetry: the evolutionary advantages of an active damage retention mechanism

    13:00-14:00

    Lunch

    14:00-16:00

    Session 7: Protein aggregation and toxicity

    Chairs: Joris Winderickx and Charles Boone

     

    Tiago Outeiro (Göttingen, Germany)

    14:00-14:20

    Inspired by Tango: in memory of Sue Lindquist

    14:20-14:50

    The yeast toolbox to determine the role of posttranslational modifications in Parkinson's disease

    14:50-15:20

    Dina Petranovic (Gothenburg, Sweden)
    Yeast models of human mutant ubiquitin (UBB+1) and amyloid β-peptide (Aβ)

    15:20-15:38

    Raffael Schaffrath (Kassel, Germany)
    Inappropriate tRNA modifications trigger protein aggregation and toxicity

    15:38-15:56

    Ju Zheng (Leuven, Belgium)
    The management of nucleocytoplasmic translocation machinery of mutant huntingtin protein in yeast

    16:00-16:30

    Coffee break

    16:30-18:00

    Session 8: Yeast models of human diseases I

    Chairs: Marie Hardwick and Tiago Outeiro

    16:30-17:00

    Joris Winderickx (Leuven, Belgium)
    On nutrient signaling, second messengers and the use of yeast as disease model

    17:00-17:30

    Richard Y. Zhao (Baltimore, MD, USA)
    The use of fission yeast as a surrogate system for the discovery of cytopathic factors through genome-wide analysis of Zika viral proteins

    17:30-17:48

    Tiziana Cervelli (Pisa, Italy)
    Identification of BRAFV600E functional interactors in Saccharomyces cerevisiae

    17:48-18:06

    Frederik Van Leemputte (Leuven, Belgium)
    Yeast and cancer: common mechanism underlying activation of Ras by glycolytic flux

    18:10-19:00

    Poster session II

    19:00-20:00

    Meeting of the IMYA Scientific Advisory Board

  • Wednesday, 17 May

    8:30-10:00

    Session 9: Yeast models of human diseases II

    Chairs: Zdena Palková and Carlo Bruschi

    8:30-9:00

    Ali Gargouri (Sfax, Tunisia)
    Cell death induced by p53 expression in yeast: detection and suppression

    9:00-9:30

    Carlo Bruschi (Salzburg, Austria)
    Apoptotic dynamics ensuing Bridge-Induced chromosome Translocation

    9:30-9:48

    Randy Strich (Stratford, NJ, USA)
    Weaponizing the mitochondria to attack cancer: applying lessons learned in yeast

    9:48-10:06

    Damiano Pellegrino Coppola (Milan, Italy)
    Fatty acid metabolism: risks for obese cells during growth

    10:06-10:24

    Carlo Marobbio (Bari, Italy)
    Impaired amino acid metabolism in a yeast model of congenital sideroblastic anemia

    10:30-11:00

    Coffee break

    11:00-13:00

    Session 10: Yeast-based drug discovery

    Chairs: Richard Y. Zhao and Maurizio Bettiga

    11:00-11:30

    Ralf Braun (Bayreuth, Germany)
    Neurotoxic 43 kDa TAR DNA-binding protein (TDP-43) interferes with its own degradation via the endolysosomal pathway

    11:30-12:00

    Paula Ludovico (Braga, Portugal)
    Supplemented metabolites as anti-aging strategies in yeast

    12:00-12:18

    Susana Chaves (Braga, Portugal)
    Yeast as a tool to understand proteasome-mediated sensitivity to the chemotherapeutic agent cisplatin

    12:18-12:36

    Maria João Sousa (Braga, Portugal)
    Novel benzo[a]phenoxazine derivative induces cell death in yeast accompanied by nuclear and vacuolar permeabilization

    12:36-12:54

    Vladimir Titorenko (Montreal, QC, Canada)
    A plant extract delays yeast chronological aging because it decelerates the age-related onset of liponecrosis, a mode of programmed cell death

    13:00-14:00

    Lunch

    15:00

    Excursion and gala dinner

    For details visit the information page (Social Events)

  • Thursday, 18 May

    9:00-11:00

    Session 11: Systems-based model of cell death and ageing processes

    Chairs: Hiroshi Takagi and Duccio Cavalieri

    9:00-9:30

    Charles Boone (Toronto, ON, Canada)
    A global genetic interaction network maps a wiring diagram of cellular function

    9:30-10:00

    Duccio Cavalieri (Florence, Italy)
    Dead yeast cells as common goods: programmed cell death as the result of cooperation and competition at the community level, a systems biology approach

    10:00-10:18

    Niek Welkenhuysen (Gothenburg, Sweden)
    A microfluidic platform to study the ageing process throughout a cell lineage

    10:18-10:36

    Sara Verbandt (Leuven, Belgium)
    Protein kinase c localizes to lipid droplets in yeast under copper stress

    10:36-10:56

    Beckman Coulter

    Carla Montesano (Rome, Italy)
    Flow Cytometry in apoptosis analysis: from human to yeast cells and back

    11:00-11:30

    Coffee break

    11:30-13:15

    Session 12: Stress responses for improvement of bioprocesses

    Chairs: Jens Nielsen and Enzo Martegani

    11:30-12:00

    Maurizio Bettiga (Gothenburg, Sweden)
    Plasma membrane as a crucial player in acetic acid effect on yeast

    12:00-12:18

    David Zhou (Montreal, QC, Canada)
    Investigating the anti-apoptotic potential of LIM-domain containing proteins in humanized Saccharomyces cerevisiae

    12:18-12:36

    Nicoletta Guaragnella (Bari, Italy)
    RTG2 modulates Hog1p phosphorylation in the evasion of acetic acid-induced programmed cell death in acid-stress adapted yeast cells

    12:36-12:54

    Rosa Santomartino (Rome, Italy)
    Deletion of KlMga2, a hypoxic regulator, reveals connection between lipid biosynthesis, ROS metabolism and longevity in Kluyveromyces lactis

    12:54-13:12

    Renata Tisi (Milan, Italy)
    Novel roles for ion transporters in cell death pathways in budding yeast

    13:15-13:30

    Closing remarks

    13:30-14:30

    Lunch and Departure

    15:00-17:00

    Meeting of the Italian yeast research community

  • Posters

    When preparing your poster, please remember that its maximum size is A0 (841 x 1189 mm; width x height). Note that the orientation is portrait, not landscape.

    Correct format... poster should be PORTRAIT  poster should NOT be LANDSCAPE Wrong format...

    Posters will be on display for the entire duration of the meeting and discussed during the two poster sessions: Monday, 15 May, and Tuesday, 16 May.

    P.1  Maria Azzopardi (Msida, Malta)
    L-glutamate partially rescues redox-compromised yeast cells from aspirin-induced apoptosis

    P.2  Xenia Chelius (Bayreuth, Germany)
    Mitochondrial dynamics affect retention of cytosolic protein aggregates in yeast mother cells

    P.3  Xin Chen (Gothenburg, Sweden)
    Physiological and transcriptome analyses of amyloid-β peptide-induced cytotoxicity in Saccharomyces cerevisiae

    P.4  Kaat De Cremer (Heverlee, Belgium)
    Identification of novel coding and non-coding differentially expressed transcripts in copper-stressed yeast

    P.5  Jana Deisel (Bayreuth, Germany)
    Identification of protein interaction partners of human neurotoxic proteins in yeast by protein fragment complementation

    P.6  Ali Gargouri (Sfax, Tunisia)
    FTIR spectroscopy of whole cells for the monitoring of yeast apoptosis mediated by p53 over-expression and its suppression by Nigella sativa extracts

    P.7  Nicoletta Guaragnella (Bari, Italy)
    Identification of active compounds for the development of new anti-prostate cancer drugs

    P.8  Tori Hallisey and Lukas Ritzer (Providence, RI, USA)
    The Parkinson’s disease protein α-synuclein alters the microenvironment of the endoplasmic reticulum of Saccharomyces cerevisiae

    P.9  Sebastian Hofer (Graz, Austria)
    A natural polyphenol counteracts aging-associated mitochondrial decline

    P.10  Katharina Kainz (Graz, Austria)
    Natural killers: dietary polyphenols as novel antifungal agents

    P.11  Róża Kucharczyk (Warsaw, Poland)
    Two cancer cancer-related mutations in ATP synthase mitochondrial ATP6 gene impact the ATP synthase dimers formation during permeability transition

    P.12  Dara Lo (Toronto, ON, Canada)
    Dying as a process – the ordered events that occur at an organellar level as cells die

    P.13  Enzo Martegani (Milan, Italy)
    Detection of PKA activity in single yeast cell, using a a FRET-based A-kinase activity reporter

    P.14  Liam McDonough (Providence, RI, USA)
    Yeast Bax Inhibitor (BXI1/YBH3) is involved in calcium and redox homeostasis of the ER in Saccharomyces cerevisiae

    P.15  James Mullin (Providence, RI, USA)
    Yeast Bxi1p/Ybh3p is a pH-sensitive calcium channel in Escherichia coli

    P.16  Christoph Ruckenstuhl (Graz, Austria)
    Receptor-independent effects of progesterone - elucidating physiological effects of progesterone treatment in a yeast model

    P.17  Belém Sampaio-Marques (Braga, Portugal)
    Coordinate regulation of autophagy and proteasome mediates chronological lifespan extension by caloric restriction

    P.18  David Seynnaeve (Heverlee, Belgium)
    Development and validation of a humanized Schizosaccharomyces pombe yeast model for the study of tau-mediated cytotoxicity

    P.19  Amit Shrestha (Ottawa, ON, Canada)
    Ubiquitin mediated regulation of Yca1 function in proteostasis

    P.20  Giulia Stamerra (Milan, Italy)
    Resveratrol supplementation at the diauxic shift increases oxidative stress and reduces yeast Chronological LifeSpan

    P.21  Carolin Sterk (Osnabrück, Germany)
    Yeast Dck1 and Lmo1 act on the small GTPase Rho5 and mediate mitophagy and apoptosis

    P.22  Mariarita Stirpe (Rome, Italy)
    Increased levels of RNA oxidation enhance the reversion frequency in Kllsm4Δ1, an ageing pro-apoptotic yeast mutant

    P.23  Libuse Vachova (Prague, Czech Republic)
    Metabolic reprogramming of biofilm yeast colonies

    P.24  Vincent Van Eyck (Kortrijk, Belgium)
    Decreased vacuolar calcium storage and impaired exocytosis confer alpha-synuclein-induced calcium dysregulation in S. cerevisiae

    P.25  Ward Vanthienen (Heverlee, Belgium)
    Identification and characterization of synthetic compounds that combined with 3-bromopyruvate specifically induce apoptosis in Saccharomyces cerevisiae

    P.26  Valentina Vapore (Rome, Italy)
    Annurca apple (M. pumila Miller cv Annurca) extracts act against stress and ageing in S. cerevisiae yeast cells